ZR Plasmid Miniprep-Classic Kits
15 min. Prep, 100 Preps/Unit
15 min. Prep, 800 Preps/Unit
- For purification of high quality, endotoxin-free plasmid DNA for restriction endonuclease digestion, sequencing, transfection, transformation, etc.
- Innovative colored P1, P2, and P3 buffers for rapid identification of complete bacterial cell lysis and neutralization steps
- Unique column design: zero buffer retention and low (30 µl) elution volume
- Processing time: 15 minutes
- Plasmid DNA size: Up to 25kb
- Yield Up to 25 µg per preparation
- For purification of high quality, endotoxin-free plasmid DNA for restriction endonuclease digestion, sequencing, transfection, transformation, etc.
- Innovative colored P1, P2, and P3 buffers for rapid identification of complete bacterial cell lysis and neutralization steps
- Unique column design: zero buffer retention and low (30 µl) elution volume
- Processing time: 15 minutes
- Plasmid DNA size: Up to 25kb
- Yield Up to 25 µg per preparation
- Extra Zymo-Spin IIN columns: Cat# 11-502N, 11-503N
- For purification of high quality, endotoxin-free plasmid DNA for restriction endonuclease digestion, sequencing, transfection, transformation, etc.
- Innovative colored P1, P2, and P3 buffers for rapid identification of complete bacterial cell lysis and neutralization steps
- Unique column design: zero buffer retention and low (30 µl) elution volume
- Processing time: 15 minutes
- Plasmid DNA size: Up to 25kb
- Yield Up to 25 µg per preparation
| Applicable For | Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, transfections, and other sensitive applications. |
| Elution Volume | ≥ 30 μl |
| Endotoxin Levels | 50 EU/μg |
| Equipment | Microcentrifuge |
| Processing Time | 15 min |
| Purity | Typical Abs260/280 ≥1.8. |
| Size Range | Up to 25 kb |
| Supplemental Info | How to Increase Plasmid Yield DOs and DON'Ts of Plasmid Purification |
| Yield | Up to 25 μg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. |
| Applicable For | Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, transfections, and other sensitive applications. |
| Elution Volume | ≥ 30 μl |
| Endotoxin Levels | 50 EU/μg |
| Equipment | Microcentrifuge |
| Processing Time | 15 min |
| Purity | Typical Abs260/280 ≥1.8. |
| Size Range | Up to 25 kb |
| Supplemental Info | How to Increase Plasmid Yield DOs and DON'Ts of Plasmid Purification |
| Yield | Up to 25 μg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. |
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
3 Product Options
Specifications:
| Quantity | 400 Preps |
| Description | 15 min. Prep |
| Qty/Unit | 400 Preps/Unit |
Specifications:
| Quantity | 100 Preps |
| Description | 15 min. Prep |
| Qty/Unit | 100 Preps/Unit |
Specifications:
| Quantity | 800 Preps |
| Description | 15 min. Prep |
| Qty/Unit | 800 Preps/Unit |
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