CAT#:
Zymo Research
ZR BAC DNA Miniprep Kits
Zymo Research, 100 Preps/Unit
Zymo Research, 25 Preps/Unit
2 product variations: View Options
Product Overview
- For spin column purification of endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc
- Innovative colored buffers for rapid identification of complete bacterial cell lysis and neutralization steps
- Unique column design: zero buffer retention and low-volume elution as little as 10µl
Product Overview
- For spin column purification of endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc
- Innovative colored buffers for rapid identification of complete bacterial cell lysis and neutralization steps
- Unique column design: zero buffer retention and low-volume elution as little as 10µl
Technical Specifications
| Applicable For | Transfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications. |
| Elution Volume | ≥ 10 μl |
| Equipment | Microcentrifuge |
| Processing Time | 15 min |
| Purity | Typical Abs 260/280 ≥ 1.8 and Abs 260/230 ≥ 2.0 |
| Size Range | Up to ~200 kb |
| Yield | Up to 10 μg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 μg per 3 ml of culture. |
Technical Specifications
| Applicable For | Transfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications. |
| Elution Volume | ≥ 10 μl |
| Equipment | Microcentrifuge |
| Processing Time | 15 min |
| Purity | Typical Abs 260/280 ≥ 1.8 and Abs 260/230 ≥ 2.0 |
| Size Range | Up to ~200 kb |
| Yield | Up to 10 μg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 μg per 3 ml of culture. |
Technical Documents
Technical Documents
Detailed Description
The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.
2 Product Options
Catalog Number:
11-309ZB
List Price:
$672.38
Your Price:
$576.32
Specifications:
| Quantity | 100 Preps |
| Description | Zymo Research |
| Qty/Unit | 100 Preps/Unit |
Catalog Number:
11-309Z
List Price:
$215.5
Your Price:
$184.72
Specifications:
| Quantity | 25 Preps |
| Description | Zymo Research |
| Qty/Unit | 25 Preps/Unit |
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