His-Spin Protein Miniprep Kits
Zymo Research, 50 Preps /Unit
Zymo Research, 10 Preps/Unit
- Fast (5 minute) method for the purification of His-tagged proteins from cell free extracts.
- Screen bacterial colonies directly on the basis of protein expression vs. plasmid DNA.
- No special instrumentation is required other than a benchtop microcentrifuge.
- Fast (5 minute) method for the purification of His-tagged proteins from cell free extracts.
- Screen bacterial colonies directly on the basis of protein expression vs. plasmid DNA.
- No special instrumentation is required other than a benchtop microcentrifuge.
| Affinity Matrix | Nickel charged agarose |
| Binding Capacity | 1 mg |
| Elution Method | Imidazole gradient |
| Elution Volume | 100 - 200 μl (150 μl is optimal) |
| Principle Of Technology | Based on a nickel-charged His-Affinity Gel (IMAC). |
| Processing Time | 5 minutes |
| Product Storage | Please store the His-Affinity Gel, His-Binding Buffer, His-Wash Buffer, and His-Elution Buffer at 4°C. The other components can be stored at room temperature. |
| Protein Purity | Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications. |
| Required Equipment | Microcentrifuge |
| Sample Type | Cell lysates or other complex protein mixtures containing His-tagged proteins. |
| Affinity Matrix | Nickel charged agarose |
| Binding Capacity | 1 mg |
| Elution Method | Imidazole gradient |
| Elution Volume | 100 - 200 μl (150 μl is optimal) |
| Principle Of Technology | Based on a nickel-charged His-Affinity Gel (IMAC). |
| Processing Time | 5 minutes |
| Product Storage | Please store the His-Affinity Gel, His-Binding Buffer, His-Wash Buffer, and His-Elution Buffer at 4°C. The other components can be stored at room temperature. |
| Protein Purity | Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications. |
| Required Equipment | Microcentrifuge |
| Sample Type | Cell lysates or other complex protein mixtures containing His-tagged proteins. |
The His-Spin Protein Miniprep™ provides researchers with a method for fast His-tagged protein purification. The easy-to-follow procedure is based on a nickel-charged His-Affinity Gel (IMAC), innovative protein purification, and uniqueFast-Spin column technology. Up to 1 mg of His-tagged protein can be purified in as little as 5 minutes and can be eluted into as little as 100 µl of the provided His-Elution Buffer. The purified protein can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE, as well as other protein based applications. The His-Spin Protein Miniprep™ has been optimized to yield maximal protein purity indices: a single protein band is often visualized following Coomassie Blue® staining of proteins in SDS-PAGE gel (see figure below). The straightforward spin-wash-elute protocol dramatically simplifies protein purification and results are obtained in minutes, not hours!
2 Product Options
Specifications:
| Quantity | 50 Preps |
| Description | Zymo Research |
| Qty/Unit | 50 Preps /Unit |
Specifications:
| Quantity | 10 Preps |
| Description | Zymo Research |
| Qty/Unit | 10 Preps/Unit |