CAT#:
Zymo Research
dsDNA Shearase Plus (Includes Matching DCC-5 Kit)
Zymo Research, 200U/Unit
Zymo Research, 50U/Unit
2 product variations: View Options
Product Overview
- The simplest method for generating random-ended dsDNA fragments. Fragment size can be controlled by adjusting the enzyme concentration
- Included DNA Clean & Concentrator-5 (DCC-5) kit allows for easy clean-up of fragmented DNA
- Fragmented DNA is ideal for downstream applications such as library construction, Next-Gen sequencing, MeDIP, MeDIP-Seq, etc
Product Overview
- The simplest method for generating random-ended dsDNA fragments. Fragment size can be controlled by adjusting the enzyme concentration
- Included DNA Clean & Concentrator-5 (DCC-5) kit allows for easy clean-up of fragmented DNA
- Fragmented DNA is ideal for downstream applications such as library construction, Next-Gen sequencing, MeDIP, MeDIP-Seq, etc
Technical Specifications
| Brand | Zymo Research Corporation |
| Concentration | 1U/µl |
| Enzyme Inactivation | Heat in activate enzyme at 65C for 5 min. |
| Storage | Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at </= -70C. |
| Unit Definition | One unit (1 U) is defined at the amount of enzyme required to convert 250ng human DNA into DNA fragments in the range of 100-500bp in 20 minutes at 42C in total reaction volume in 10µl. |
Technical Specifications
| Brand | Zymo Research Corporation |
| Concentration | 1U/µl |
| Enzyme Inactivation | Heat in activate enzyme at 65C for 5 min. |
| Storage | Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at </= -70C. |
| Unit Definition | One unit (1 U) is defined at the amount of enzyme required to convert 250ng human DNA into DNA fragments in the range of 100-500bp in 20 minutes at 42C in total reaction volume in 10µl. |
Technical Documents
Technical Documents
Detailed Description
dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5'-phosphates and 3'-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs thus minimizing sample loss.
2 Product Options
Catalog Number:
11-709
List Price:
$1495.62
Your Price:
$1281.97
Specifications:
| Units | 200 Units |
| Description | Zymo Research |
| Qty/Unit | 200 U/Unit |
Catalog Number:
11-709B
List Price:
$422.4
Your Price:
$362.06
Specifications:
| Units | 50 Units |
| Description | Zymo Research |
| Qty/Unit | 50 U/Unit |
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