CAT#:
Cytiva
Cytiva® HisTrap™ Fast Flow
Prepacked Columns, 5 x 5 mL/Unit
Prepacked Columns, 5 x 1 mL/Unit
2 product variations: View Options
Product Overview
- High binding capacity, approx. 40 mg/ml medium
- Negligible leakage of Ni2+
- Prepacked columns offer reliable and convenient timesaving purification of histidine-tagged recombinant proteins
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
Product Overview
- High binding capacity, approx. 40 mg/ml medium
- Negligible leakage of Ni2+
- Prepacked columns offer reliable and convenient timesaving purification of histidine-tagged recombinant proteins
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
Technical Documents
Detailed Description
Ni Sepharose 6 Fast Flow consists of 9NA µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap™ IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.
2 Product Options
Catalog Number:
84-201
List Price:
$1031.4
Your Price:
$812.8
Specifications:
| Pack Size | 5 x 5 mL |
| Description | Prepacked Columns |
| Qty/Unit | 5 x 5ml/Unit |
Catalog Number:
84-200
List Price:
$297.7
Your Price:
$235.85
Specifications:
| Pack Size | 5 x 1 mL |
| Description | Prepacked Columns |
| Qty/Unit | 5 x 1ml/Unit |
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