A chemically defined, protein-free ADCF cell culture medium. HyClone CDM4CHO is a protein-free and animal-derived component-free (ADCF) cell culture medium. This regulatory-friendly medium is developed to increase process yields for the industrial manufacture of recombinant proteins using a variety of CHO cell clones. CDM4CHO medium has been successfully tested in a variety of culture systems, including T-flasks, shaker flasks, and bioreactors including fed-batch and perfusion culturing. CDM4CHO medium is available in liquid and powder formats in user-friendly packaging.