PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1 ml can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation. Instructions supplied with the columns.