CNBr-activated Sepharose 4 Fast Flow is based on the well established Sepharose Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. CNBr-activated Sepharose 4 Fast Flow is designed for multipoint attachment of protein ligands containing amino groups. The preparation and use of affinity chromatography media by coupling biospecific ligands to CNBr-activated Sepharose 4 Fast Flow is a widely used, successful and well-documented approach, with an easy, rapid and efficient coupling procedure.