Tween® 20 is a nonionic surfactant and detergent widely applied in biochemical and life science research. Its predominant use involves the solubilization of membrane proteins during the isolation of membrane-protein complexes. Moreover, it serves as a component in various washing, blocking, dilution, and stabilization buffers. Additionally, it functions as a reagent for immunohistochemistry, encompassing techniques like Western blotting and ELISAs. In these applications, Tween 20 plays crucial roles such as preventing non-specific antibody binding, minimizing background staining, and ensuring the even distribution of reagents.
