Acrylamide (C₃H₅NO) is a water-soluble monomer (71.08 g/mol) that forms polyacrylamide gels used in electrophoresis to separate proteins and nucleic acids. It polymerizes with bis-acrylamide and initiators like APS and TEMED. Common applications in life science labs include SDS-PAGE, Western blotting, and nucleic acid analysis.