GC5 Chemically Competent cells from Genesee Scientific are appropriate for general cloning, plasmid propagation, construction of gene banks, generation of cDNA libraries, mutagenesis and routine subcloning. They carry the tonA genotype that confers resistance to T1 and T5 phage, which offers added security to protect valuable clones and libraries. Cells provide a-complementation of the b-galactosidase gene for blue/white color screening. recA1 and endA1 markers minimize unwanted recombination and enhance plasmid quality. Efficient transformation of unmethylated DNA from PCR applications (hsdR).
These cells are resistant to bacteriophage T1 which attacks E. coli and spreads rapidly by aerosolization. The T1 resistant competent cells protect samples from possible bacteriophage T1 infection.
