Intact Genomics igMaxTM DH10B derivative ElectroCompetent cells are suitable for demanding cloning situations, such as synthetic bio-applications, BAC cloning, assembling large and multi-DNA fragments, and large construct transformation or cloning difficult targets, requiring the greatest number of transformants possible. Utilizing proprietary manufacturing methods, these cells allow for effective transformation of all large DNA molecules (?10kb up to 350kb!).
Transformation efficiency is tested by using pUC19, ~50kb, and >100kb plasmids. The pUC19 control DNA is supplied with the kit and using the protocol given below. Transformation efficiency should be ?5 x 1010 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.
