| Additional Info | Partly restriction-deficient; good strain for cloning repetitive DNA (recA-). Suppresses many amber mutations when glutamine is available but not the S100 or S7 mutation of λ, e.g.,λgt11. Can be used for M13 cloning/sequencing and blue/white screening. |
| Genotype | F`[traD36 proA+B+ laclq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rk- mk+) relA1 recA1 |
| Processing Time | 20 Seconds |
| Product Storage | -70°C to -80°C |
| Supplemental Info | 3 Things to Know for Making Competent Cells |
| Transformation Efficiency | 108 - 109 transformants per μg of plasmid DNA |
