(11-465HA) 5-mC DNA ELISA Kit Zymo Research 2x96/Unit
(11-465HA) 5-mC DNA ELISA Kit Zymo Research 2x96/Unit
CAT#: 11-465HA Zymo Research

5-mC DNA ELISA Kit

Zymo Research, 2x96/Unit

List Price: $1,389.78
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$1,191.25
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Product Overview
  • For high-throughput detection of global 5-methylcytosine (5-mC) in DNA
  • The streamlined workflow can be completed in less than 3 hours
Technical Specifications
Detection> 0.5% 5-mC per 100 ng DNA
EquipmentIncubator and ELISA plate reader. A multi-channel pipettor is recommended. An automated plate washer may be used for blocking and wash steps.
InputThis protocol is optimized for 100 ng input DNA/well. Compatible with DNA in the range of 10-200 ng/well.
Sample SourcePurified genomic DNA, plasmid DNA, PCR amplification products, or DNA fragments in water, Tris-EDTA, or similar.
Detailed Description
The 5-mC DNA ELISA Kit allows researchers to accurately quantitate 5-mC in any DNA sample in less than 3 hours. The kit features an Anti-5-Methylcytosine monoclonal antibody that is both sensitive and specific for 5-mC. The assay is compatible with a wide range of input DNA from vertebrate, plant, and microbial sources as well as methylated PCR amplicons and fragmented DNA. Percent 5-mC in a DNA sample can be accurately quantified from a standard curve generated with specially designed controls included with the kit. Also, the fast, streamlined workflow is ideal for high-throughput analyses.

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