Applicable For | All sensitive downstream applications such as qPCR and Next-Generation sequencing. |
Equipment | Microcentrifuge, heat block/bath (55°C and 90°C), isopropanol, beta-mercaptoethanol (optional). |
Processing Volume | Tissue - ≤25 mg or (4) 20 μm thick with a surface area of 20 mm2 |
Purity | Typical A260/A280 ≥ 1.8 |
Sample Source | Tissue from paraffin block or tissue sections. Compatible with fresh/frozen tissue specimens |
Sample Storage | Eluted DNA should be stored at ≤ -20°C. |
Size Range | > 50 bp |
Supplemental Info | Purpose of Genomic DNA Extraction & Tips For Success |
Type | Total DNA |
Yield | Up to 25 μg total DNA can be eluted into ≥ 50 μl. |