Applicable For | For all sensitive downstream applications, such as Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc. |
Elution Volume | ≥ 6 μl |
Equipment | Microcentrifuge, vortex, cell disrupter/pulverizer |
Processing Time | ≤ 10 minutes |
Processing Volume | 10-20 mg (wet weight) fungi or bacteria, 2x108 bacterial cells, or 2x107 yeast cells |
Purity | RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc.
A260/A280, A260/A230: > 1.8. |
Sample Source | Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled. |
Size Range | Total RNA ≥ 17 nt |
Type | Total RNA |
Yield | Microprep
10 μg RNA (binding capacity)
≥ 6 μl (elution volume) |