CAT#:
20-313
Prometheus Protein Biology Products
OneBlock™ Western-CL Blocking Buffer
Enhancing & Blocking Solution, 1000ml/Unit
Product Overview
- Antibody-antigen signal enhancing blocking solution and antibody incubation solution for chemiluminescent Western Blots
- Enhanced the Western blot signal compared to non-fat dry milk with 20 common antibodies and different sample types
- Decreased background - Decreased non-specific bands and lowered background
- Sensitivity - Get better signals for low protein sample concentrations
- Convenience - Pre-made buffer means you save preparation and optimization time
- Provided as a convenient ready-to-use 1x solution intended to directly replace other commonly used blocking buffers
- Stable at 4ºC for at least one year. Unopened bottles stable at room temperature for up to 6 months
- Block the membrane for one hour at ambient temperature with gentle agitation using a sufficient volume of buffer to completely cover the membrane
Detailed Description
OneBlock™ Western-CL is a novel blocking solution, optimized to enhance specific antibody-antigen interactions for chemiluminescent Western Blots. This all-in-one blocking and antibody incubation solution is designed to improve sensitivity and decrease overall background. Non-specific binding caused by low quality antibodies is reduced while signal from the specific antibody-antigen complex is stabilized and enhanced. Provided as a convenient ready-to-use solution intended to directly replace other commonly used blocking buffers for Western Blotting.
No need to add sodium azide as OneBlock contains ProClin 300 to prevent microbial growth. In addition, sodium azide should be avoided as it inhibits HRP activity.
Citations
Powered by Bioz