2 product variations: View Options
Product Overview
- The simplest method for generating random-ended dsDNA fragments
- Fragment size can be controlled by adjusting the enzyme concentration
- Fragments generated using dsDNA Shearase Plus are ideal for library construction, NGS, methylated DNA immunopreciptation (MeDIP, MeDIP-Seq), etc
Product Overview
- The simplest method for generating random-ended dsDNA fragments.
- Fragment size can be controlled by adjusting the enzyme concentration.
- Fragments generated using dsDNA Shearase Plus are ideal for library construction, Next-Gen sequencing, methylated DNA immunopreciptation (MeDIP, MeDIP
Technical Specifications
| Brand | Zymo Research Corporation |
| Concentration | 1U/µl |
| Enzyme Inactivation | Heat inactivate enzyme at 65°C for 5 min. |
| Storage | Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at </= -70°C. |
| Unit Definition | One unit (1 U) is defined at the amount of enzyme required to convert 250ng human DNA into DNA fragments in the range of 100-500bp in 20 minutes at 42°C in total reaction volume in 10µl. |
Technical Specifications
| Concentration | 1 U/μl |
| Enzyme Inactivation | Heat inactivate enzyme at 65°C for 5 min. |
| Storage | Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70°C. |
| Unit Definition | One unit (1 U) is defined at the amount of enzyme required to convert 250 ng human DNA into DNA fragments in the range of 100-500 bp in 20 minutes at 42°C in total reaction volume in 10 μl. |
Technical Documents
Detailed Description
dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs, thus minimizing sample loss.
2 Product Options
Catalog Number:
11-708
List Price:
$260.63
Your Price:
$223.4
Specifications:
| Units | 50 Units |
| Description | Zymo Research |
| Qty/Unit | 50 U/Unit |
Catalog Number:
11-648
List Price:
$914.49
Your Price:
$783.85
Specifications:
| Units | 200 Units |
| Description | Zymo Research |
| Qty/Unit | 200 U/Unit |
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