CAT#:
Zymo Research
EZ DNA Methlyation-Lightning Kits
Shallow-Well, 2 x 96 Rxns/Unit
MagPrep, 8 x 96 Rxns/Unit
Zymo Research, 200 Rxns/Unit
MagPrep, 4 x 96 Rxns/Unit
Deep-Well, 2 x 96 Rxns/Unit
Zymo Research, 10 Rxns/Unit
Zymo Research, 50 Rxns/Unit
Zymo Research, 200 Rxns/Unit
8 product variations: View Options
Product Overview
- Fastest method for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis.
- Ready-to-use conversion reagent is added directly to DNA.
- High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen sequencing.
- Conversion Efficiency: >99.5% of non-methylated cytosine residues are converted to uracil; >99.5% protection of methylated cytosines.
- Optimal DNA Input: Samples containing between 100 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng.
Product Overview
- High-throughput procedure for automated rapid and complete bisulfite conversion of DNA for methylation analysis.
- Ready-to-use conversion reagent is added directly to DNA.
- High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen sequencing.
- Conversion Efficiency: >99.5% of non-methylated cytosine residues are converted to uracil; >99.5% protection of methylated cytosines.
- Optimal DNA Input: Samples containing between 100 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng.
Product Overview
- Fastest method for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis.
- Ready-to-use conversion reagent is added directly to DNA.
- High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen sequencing.
- Conversion Efficiency: >99.5% of non-methylated cytosine residues are converted to uracil; >99.5% protection of methylated cytosines.
- Optimal DNA Input: Samples containing between 100 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng.
- CE-IVD
Product Overview
- High-throughput procedure for automated rapid and complete bisulfite conversion of DNA for methylation analysis.
- Ready-to-use conversion reagent is added directly to DNA.
- High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen sequencing.
- Conversion Efficiency: >99.5% of non-methylated cytosine residues are converted to uracil; >99.5% protection of methylated cytosines.
- Optimal DNA Input: Samples containing between 100 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng.
Product Overview
- Fastest method for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis.
- Ready-to-use conversion reagent is added directly to DNA.
- High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen sequencing.
- Conversion Efficiency: >99.5% of non-methylated cytosine residues are converted to uracil; >99.5% protection of methylated cytosines.
- Optimal DNA Input: Samples containing between 100 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng.
Product Overview
- Fastest method for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis.
- Ready-to-use conversion reagent is added directly to DNA.
- High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen sequencing.
- Conversion Efficiency: >99.5% of non-methylated cytosine residues are converted to uracil; >99.5% protection of methylated cytosines.
- Optimal DNA Input: Samples containing between 100 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng.
Product Overview
- Fastest method for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis.
- Ready-to-use conversion reagent is added directly to DNA.
- High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen sequencing.
- Conversion Efficiency: >99.5% of non-methylated cytosine residues are converted to uracil; >99.5% protection of methylated cytosines.
- Optimal DNA Input: Samples containing between 100 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng.
Product Overview
- Fastest method for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis.
- Ready-to-use conversion reagent is added directly to DNA.
- High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen sequencing.
- Conversion Efficiency: >99.5% of non-methylated cytosine residues are converted to uracil; >99.5% protection of methylated cytosines.
- Optimal DNA Input: Samples containing between 100 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng.
Technical Specifications
| Conversion | > 99.5% |
| Elution Volume | Deep-well: ≥ 15 μl Shallow-well: ≥ 30 μl |
| Equipment | Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers. |
| Input | 100 pg - 2 μg of DNA. |
| Processing Time | 1.5 hours |
| Recovery | > 80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | > 99.5% |
| Elution Volume | ≥ 25 μl |
| Equipment | Thermocycler with heated lid, heating element for 96-well plate, magnetic stand. |
| Input | 100 pg - 2 μg of DNA. |
| Processing Time | 1.5 hours |
| Recovery | > 80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | > 99.5% |
| Elution Volume | ≥ 10 μl |
| Equipment | Thermocycler with heated lid and microcentrifuge. |
| Input | 100 pg - 2 μg of DNA. |
| Processing Time | 1.5 hours |
| Recovery | > 80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | > 99.5% |
| Elution Volume | ≥ 25 μl |
| Equipment | Thermocycler with heated lid, heating element for 96-well plate, magnetic stand. |
| Input | 100 pg - 2 μg of DNA. |
| Processing Time | 1.5 hours |
| Recovery | > 80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | > 99.5% |
| Elution Volume | Deep-well: ≥ 15 μl Shallow-well: ≥ 30 μl |
| Equipment | Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers. |
| Input | 100 pg - 2 μg of DNA. |
| Processing Time | 1.5 hours |
| Recovery | > 80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | > 99.5% |
| Elution Volume | ≥ 10 μl |
| Equipment | Thermocycler with heated lid and microcentrifuge. |
| Input | 100 pg - 2 μg of DNA. |
| Processing Time | 1.5 hours |
| Recovery | > 80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | > 99.5% |
| Elution Volume | ≥ 10 μl |
| Equipment | Thermocycler with heated lid and microcentrifuge. |
| Input | 100 pg - 2 μg of DNA. |
| Processing Time | 1.5 hours |
| Recovery | > 80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | > 99.5% |
| Elution Volume | ≥ 10 μl |
| Equipment | Thermocycler with heated lid and microcentrifuge. |
| Input | 100 pg - 2 μg of DNA. |
| Processing Time | 1.5 hours |
| Recovery | > 80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Documents
Technical Documents
Technical Documents
Technical Documents
Detailed Description
The EZ-96 DNA Methylation-Lightning™ Kit features high-throughput (96-well) bisulfite treatment and conversion of DNA for methylation analysis. Key to the fast workflow is the ready-to-use Lightning Conversion Reagent. No preparation is necessary, simply add this unique reagent to a DNA sample, wait about an hour, and let the reaction proceed to completion. DNA denaturation and bisulfite conversion processes are combined with added heat to facilitate rapid denaturation. Desulphonation and clean-up of the converted DNA is performed using a unique 96-well spin-plate. High yield, converted DNA is ideal for PCR, array, bisulfite and next generation sequencing, etc.
Citations
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8 Product Options
Catalog Number:
11-338S
List Price:
$1182.94
Your Price:
$1013.94
Specifications:
| Quantity | 2 x 96 Reactions - Shallow Well |
| Description | Shallow-Well |
| Qty/Unit | 2 x 96 Rxns/Unit |
Catalog Number:
11-338MB
List Price:
$3272.97
Your Price:
$2805.4
Specifications:
| Quantity | 8 x 96 Reactions - MagPrep |
| Description | MagPrep |
| Qty/Unit | 8 x 96 Rxns/Unit |
Catalog Number:
11-338E
List Price:
$1286.1
Your Price:
$1102.37
Specifications:
| Quantity | 200 Reactions, CE/IVD |
| Description | Zymo Research |
| Qty/Unit | 200 Rxns/Unit |
Catalog Number:
11-338M
List Price:
$1938.53
Your Price:
$1661.6
Specifications:
| Quantity | 4 x 96 Reactions - MagPrep |
| Description | MagPrep |
| Qty/Unit | 4 x 96 Rxns/Unit |
Catalog Number:
11-338D
List Price:
$1002.44
Your Price:
$859.24
Specifications:
| Quantity | 2 x 96 Reactions - Deep Well |
| Description | Deep-Well |
| Qty/Unit | 2 x 96 Rxns/Unit |
Catalog Number:
11-338AB
List Price:
$89.95
Your Price:
$77.1
Specifications:
| Quantity | 10 Reactions |
| Description | Zymo Research |
| Qty/Unit | 10 Rxns/Unit |
Catalog Number:
11-338
List Price:
$941.87
Your Price:
$807.32
Specifications:
| Quantity | 50 Reactions |
| Description | Zymo Research |
| Qty/Unit | 50 Rxns/Unit |
Catalog Number:
11-338B
List Price:
$1240.58
Your Price:
$1063.36
Specifications:
| Quantity | 200 Reactions |
| Description | Zymo Research |
| Qty/Unit | 200 Rxns/Unit |
| Catalog # | Description | Qty/Unit | Quantity | Price (USD) | Quantity | Add to Order | |
|---|---|---|---|---|---|---|---|
| 11-338 | Zymo Research | 50 Rxns/Unit | 50 Reactions | ||||
| 11-338AB | Zymo Research | 10 Rxns/Unit | 10 Reactions | ||||
| 11-338B | Zymo Research | 200 Rxns/Unit | 200 Reactions | ||||
| 11-338D | Deep-Well | 2 x 96 Rxns/Unit | 2 x 96 Reactions - Deep Well | ||||
| 11-338E | Zymo Research | 200 Rxns/Unit | 200 Reactions, CE/IVD | ||||
| 11-338M | MagPrep | 4 x 96 Rxns/Unit | 4 x 96 Reactions - MagPrep | ||||
| 11-338MB | MagPrep | 8 x 96 Rxns/Unit | 8 x 96 Reactions - MagPrep | ||||
| 11-338S | Shallow-Well | 2 x 96 Rxns/Unit | 2 x 96 Reactions - Shallow Well |
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