Fluorescein-labeled proaerolysin (FLAER) is used in flow cytometry to diagnose paroxysmal nocturnal hemoglobinuria (PNH). It is a unique protein that binds tightly and specifically to mammalian glycol-phosphatidylinositol (GPI) anchored proteins on the cell surface. FLAER fails to bind to lymphocytes, monocytes, and granulocytes in a PNH patient due to the patient's white blood cells losing expression of the GIP anchored cell-surface protein. In addition to antibody-mediated PNH cell detection, FLAER detects ALL GPI-anchored proteins expressed on the surface of leukocytes.
mFluor™ fluorescent labeling dyes are developed specifically for flow cytometry applications to enable optimal multi-color detection. These dyes are excellent alternatives to the phycoprotein-based tandems that are quite difficult to couple to an antibody or other biomolecules and offer greater light stability and brighter fluorescence. The mFluor™ 450 fluorophore is a great alternative to these dyes: V450, Pacific Blue™, VioBlue®, eFluor® 450, Brilliant Violet 421 and Super Bright 436.
