1M HEPES Buffer, pH 7.3

HEPES Buffer is a buffering solution used in cell culture media and biological research. It is used to maintain physiological pH of cells despite changes in the level of carbon dioxide produced during aerobic respiration. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent at most biological pH’s. HEPES buffer is categorized as an all-purpose buffer for biological research.