MTC Bio’s Gel Crusher™ Spin Columns are designed to replace labor-intensive gel fragmentation steps (crush and soak method, etc.). Commonly used in molecular cloning, PCR, restriction digestions, sequencing, and labeling protocols, these spin columns can be used to fragment gel slices (typically cut with a gel extractor or razor blade) into smaller pieces. These smaller uniform pieces will produce greater yields and can be easily pipetted using wide bore tips into an NaCl solution. This reduces the time to create a fine slurry for further elution, filtration, incubation, etc. Place the Gel Crusher™ column in the 2ml receiver tube and centrifuge the column/tube assembly in a standard benchtop microcentrifuge for 5 minutes at maximum speed (approximately 15,000×g).The gel will be forced through the 600µm pores into the 2ml receiver tube. The crushed fragments will be small enough to be aspirated/dispensed through a pipette tip.
