| Elution Volume | cfDNA and cfRNA can be eluted into 15 μl (to maximize total yield) or as little as 6 μl (for highly concentrated eluate) of DNase/RNase-Free Water. |
| Processing Time | Typically requires 30 minutes (cfDNA/cfRNA co-elution protocol) to 45 minutes (cfDNA/cfRNA separation protocol) hands-on time to process 10 samples. Sample digestion requires 2 hours. |
| Purity | High quality cell-free DNA (cfDNA) and cell-free RNA (cfRNA) are ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing. |
| Required Equipment | Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold. |
| Sample Types | Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids. |
| Size | DNA ≥ 50 bp and RNA ≥ 17 nt. |
| Yield | Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum for both cfDNA and cfRNA. Kit is optimized for recovery of total cfDNA and cfRNA, including small RNA and microRNA. |
