| Autolysis | Lyses easily. The parent strain JM109 itself will release about 20% of cellular protein after one freeze-thaw cycle. This strain will lyse in a wide range of buffer conditions. 80-90% of E. coli are lysed after a single freeze-thaw treatment. |
| Cell Growth | Grows well, especially when medium is supplemented with 1 mM Mg2+ |
| DNA Extraction | This strain is EndA- and yields high quality DNA preparations. |
| DNA Stability | The RecA- mutation in XJa stabilizes repetitive DNA sequences. |
| Genotype | F`[traD36 proA+B+ laclq ∆(lacZ)M15] ∆(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rK- mK+) relA1 recA1 ΔaraB::λR, cat (CmR) |
| Processing Time | 10 minutes |
| Product Storage | -70°C to -80°C |
| Protein Expression | Suitable for general screening, but proteases may degrade small or otherwise unstable recombinant proteins. |
