Intact Genomics (ig®) AGL-1 (pSoup) ElectroCompetent Agrobacterium cells are optimized for the highest transformation efficiencies, ideal for applications with cDNA or gDNA library construction.  The AGL1 (pSoup) strain has resistance to rifampicin, carbenicillin, and streptomycin (see genotype). The Ti plasmid pTiBo542DT-DNA has the T-DNA region deleted and transformed with a binary vector containing the missing T-region restores the T-DNA binary system function. The pSoup plasmid aids in the replication of pGreen, 62SK, and pGs2 series plasmids. This system is used for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.

Transformation efficiency is tested by using the pCAMBIA1391z control DNA supplied with the kit and using the protocol in this manual. Transformation efficiency should be ?1 x 107 CFU/µg pCAMBIA1391z DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.