Cat #: 11-322HB
Zymo Research D6011-FM Quick-DNA Fecal/Soil Microbe 96 Magbead Kit, Zymo Research, 2 x 96 Preps/Unit
Cat #: 11-322HB
Zymo Research D6011-FM Quick-DNA Fecal/Soil Microbe 96 Magbead Kit, Zymo Research, 2 x 96 Preps/Unit
Zymo Research
2 x 96 Preps/Unit
Brand: Zymo Research- Rapid, high-throughput method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples, including those from humans, rats, cattle, etc
- Ultra-high density BashingBeads are fracture resistant and chemically inert
- Zymo MagBinding Bead technology effectively removes PCR inhibitors from the DNA product
$800.05
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Zymo Research
2 x 96 Preps/Unit
Brand: Zymo Research- Rapid, high-throughput method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples, including those from humans, rats, cattle, etc
- Ultra-high density BashingBeads are fracture resistant and chemically inert
- Zymo MagBinding Bead technology effectively removes PCR inhibitors from the DNA product
The Quick-DNA Fecal/Soil Microbe 96 Magbead Kits are designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in as little as 90 minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo MagBinding Bead technology, which features Zymo Research's Inhibitor Removal technology, is then used to isolate the DNA. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.
| Brand | Zymo Research Corporation |
|---|---|
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | >/= 37.5µl |
| Equipment | Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block, 2ml 96 well plates and reagent carriers (user supplied). |
| Purity | A260/A280nm >/=1.8. |
| Sample Source | Feces or soil |
| Sample Storage | DNA stored at </= -20°C. |
| Size Range | Capable of recovering genomic DNA up to and above 40kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Type | Total DNA |
| Yield | </= 25µg total DNA |
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Q1: Are there any tips in optimzing bead beating conditions?
We have validated our kits with both high-speed homgenizers and low-speed disruptors. We highly recommend users to optimize their bead beating conditions for their own instruments. We recommend using a 2 ml-tube adapter to ensure that the bead beating is efficent (do not use adapters made of foam). For high-speed homogenizers, we recommend a total of 5 mins bead beating (1 min interval at 6.5 m/s with 5 mins rest, repeat 5 times). For low-speed cell disruptors, we recommend 30 mins at max speed.
Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). However, if bead beating is optimized and lysis is efficient, the addition of BME is not necessary and can be omitted.
No additional RNase A treatment is required when processing samples within kit capacity. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through.
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